Biography Our laboratory seeks to understand how cells sense and respond to their external environment. In this endeavor, we study the numerous proteins that lie at the cellular surface that enable individual cells to decipher the enormous number of stimuli that coordinate normal physiology. To gain a fundamental understanding of such transmembrane signaling processes, we use a diverse array of techniques spanning biochemical and biophysical interrogation to protein engineering. Our ultimate goal is to use these insights to discover new approaches to precisely control cellular signaling. Role Faculty Category Clinical Education M.D., 05/2016 - , Stanford UniversityPh.D., 02/2014 - Biophysics, Stanford UniversityB.A., 05/2008 - Chemistry and Biology, Washington University in St. Louis Publications Phenotypic pleiotropy of missense variants in human B cell confinement receptor P2RY8. LaFlam TN, Billesbølle CB, Dinh T, Wolfreys FD, Lu E, Matteson T, An J, Xu Y, Singhal A, Brandes N, Ntranos V, Manglik A, Cyster JG, Ye CJ . Cell genomics . Sep 9, 2025 Docking 14 Million Virtual Isoquinuclidines against the µ and ? Opioid Receptors Reveals Dual Antagonists-Inverse Agonists with Reduced Withdrawal Effects. Vigneron SF, Ohno S, Braz J, Kim JY, Kweon OS, Webb C, Billesbølle CB, Srinivasan K, Bhardwaj K, Irwin JJ, Manglik A, Basbaum AI, Ellman JA, Shoichet BK . ACS Central Science . Apr 29, 2025 Phenotypic pleiotropy of missense variants in human B cell-confinement receptor P2RY8. LaFlam TN, Billesbølle CB, Dinh T, Wolfreys FD, Lu E, Matteson T, An J, Xu Y, Singhal A, Brandes N, Ntranos V, Manglik A, Cyster JG, Ye CJ . bioRxiv : the preprint server for biology . Mar 6, 2025 Docking 14 million virtual isoquinuclidines against the mu and kappa opioid receptors reveals dual antagonists-inverse agonists with reduced withdrawal effects. Vigneron SF, Ohno S, Braz J, Kim JY, Kweon OS, Webb C, Billesbølle C, Bhardwaj K, Irwin J, Manglik A, Basbaum AI, Ellman JA, Shoichet BK . bioRxiv : the preprint server for biology . Jan 14, 2025 Molecular basis of proton sensing by G protein-coupled receptors. Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbølle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A . Cell . Dec 26, 2024 Role of the G protein-coupled receptor kinase 2/3 N terminus in discriminating the endocytic effects of opioid agonist drugs. Li J, Inoue A, Manglik A, von Zastrow M . Molecular pharmacology . Nov 22, 2024 Engineered odorant receptors illuminate the basis of odour discrimination. de March CA, Ma N, Billesbølle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H . Nature . Oct 30, 2024 Structural basis of μ-opioid receptor targeting by a nanobody antagonist. Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M . Nature communications . Oct 9, 2024 Molecular basis of proton-sensing by G protein-coupled receptors. Howard MK, Hoppe N, Huang XP, Macdonald CB, Mehrota E, Grimes PR, Zahm A, Trinidad DD, English J, Coyote-Maestas W, Manglik A . bioRxiv : the preprint server for biology . Apr 18, 2024 GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway. Hoppe N, Harrison S, Hwang SH, Chen Z, Karelina M, Deshpande I, Suomivuori CM, Palicharla VR, Berry SP, Tschaikner P, Regele D, Covey DF, Stefan E, Marks DS, Reiter JF, Dror RO, Evers AS, Mukhopadhyay S, Manglik A . Nature structural & molecular biology . Feb 7, 2024 Structural Basis of μ-Opioid Receptor-Targeting by a Nanobody Antagonist. Yu J, Kumar A, Zhang X, Martin C, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M . bioRxiv : the preprint server for biology . Dec 7, 2023 Engineered odorant receptors illuminate structural principles of odor discrimination. de March CA, Ma N, Billesbølle CB, Tewari J, Del Torrent CL, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H . bioRxiv : the preprint server for biology . Nov 17, 2023 Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets. Chung DH, Kong S, Young NJ, Chuo SW, Shiah JV, Connelly EJ, Rohweder PJ, Born A, Manglik A, Grandis JR, Johnson DE, Craik CS . Communications biology . Oct 12, 2023 Structure-based discovery of conformationally selective inhibitors of the serotonin transporter. Singh I, Seth A, Billesbølle CB, Braz J, Rodriguiz RM, Roy K, Bekele B, Craik V, Huang XP, Boytsov D, Pogorelov VM, Lak P, O'Donnell H, Sandtner W, Irwin JJ, Roth BL, Basbaum AI, Wetsel WC, Manglik A, Shoichet BK, Rudnick G . Cell . May 2, 2023 Structural basis of odorant recognition by a human odorant receptor. Billesbølle CB, de March CA, van der Velden WJC, Ma N, Tewari J, Del Torrent CL, Li L, Faust B, Vaidehi N, Matsunami H, Manglik A . Nature . Mar 15, 2023 Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling. Barsi-Rhyne B, Manglik A, von Zastrow M . eLife . Oct 17, 2022 Autoantibody mimicry of hormone action at the thyrotropin receptor. Faust B, Billesbølle CB, Suomivuori CM, Singh I, Zhang K, Hoppe N, Pinto AFM, Diedrich JK, Muftuoglu Y, Szkudlinski MW, Saghatelian A, Dror RO, Cheng Y, Manglik A . Nature . Aug 8, 2022 Fusion protein strategies for cryo-EM study of G protein-coupled receptors. Zhang K, Wu H, Hoppe N, Manglik A, Cheng Y . Nature communications . Jul 28, 2022 Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody. Deshpande I, Manglik A . Methods in molecular biology (Clifton, N.J.) . Jan 1, 2022 Selective G protein signaling driven by substance P-neurokinin receptor dynamics. Harris JA, Faust B, Gondin AB, Dämgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A . Nature chemical biology . Oct 28, 2021 CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes. Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang G, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, Owens TW, Peters JK, Rizo AN, Schulze-Gahmen U, Smith AM, Young ID, Yu Z, Asarnow D, Billesbølle C, Campbell MG, Chen J, Chen KH, Chio US, Dickinson MS, Doan L, Jin M, Kim K, Li J, Li YL, Linossi E, Liu Y, Lo M, Lopez J, Lopez KE, Mancino A, Moss FR, Paul MD, Pawar KI, Pelin A, Pospiech TH, Puchades C, Remesh SG, Safari M, Schaefer K, Sun M, Tabios MC, Thwin AC, Titus EW, Trenker R, Tse E, Tsui TKM, Wang F, Zhang K, Zhang Y, Zhao J, Zhou F, Zhou Y, Zuliani-Alvarez L, QCRG Structural Biology Consortium, Agard DA, Cheng Y, Fraser JS, Jura N, Kortemme T, Manglik A, Southworth DR, Stroud RM, Swaney DL, Krogan NJ, Frost A, Rosenberg OS, Verba KA . Research square . May 19, 2021 CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes. Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang G, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, Owens TW, Peters JK, Rizo AN, Schulze-Gahmen U, Smith AM, Young ID, Yu Z, Asarnow D, Billesbølle C, Campbell MG, Chen J, Chen KH, Chio US, Dickinson MS, Doan L, Jin M, Kim K, Li J, Li YL, Linossi E, Liu Y, Lo M, Lopez J, Lopez KE, Mancino A, Moss FR, Paul MD, Pawar KI, Pelin A, Pospiech TH, Puchades C, Remesh SG, Safari M, Schaefer K, Sun M, Tabios MC, Thwin AC, Titus EW, Trenker R, Tse E, Tsui TKM, Wang F, Zhang K, Zhang Y, Zhao J, Zhou F, Zhou Y, Zuliani-Alvarez L, QCRG Structural Biology Consortium, Agard DA, Cheng Y, Fraser JS, Jura N, Kortemme T, Manglik A, Southworth DR, Stroud RM, Swaney DL, Krogan NJ, Frost A, Rosenberg OS, Verba KA . bioRxiv : the preprint server for biology . May 11, 2021 Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia. Asarnow D, Wang B, Lee WH, Hu Y, Huang CW, Faust B, Ng PML, Ngoh EZX, Bohn M, Bulkley D, Pizzorno A, Ary B, Tan HC, Lee CY, Minhat RA, Terrier O, Soh MK, Teo FJ, Yeap YYC, Seah SGK, Chan CEZ, Connelly E, Young NJ, Maurer-Stroh S, Renia L, Hanson BJ, Rosa-Calatrava M, Manglik A, Cheng Y, Craik CS, Wang CI . Cell . Apr 24, 2021 Protein design and variant prediction using autoregressive generative models. Shin JE, Riesselman AJ, Kollasch AW, McMahon C, Simon E, Sander C, Manglik A, Kruse AC, Marks DS . Nature communications . Apr 23, 2021 Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits. Arveseth CD, Happ JT, Hedeen DS, Zhu JF, Capener JL, Klatt Shaw D, Deshpande I, Liang J, Xu J, Stubben SL, Nelson IB, Walker MF, Kawakami K, Inoue A, Krogan NJ, Grunwald DJ, Hüttenhain R, Manglik A, Myers BR . PLoS biology . Apr 22, 2021 Surface Proteomics Reveals CD72 as a Target for In Vitro-Evolved Nanobody-Based CAR-T Cells in KMT2A/MLL1-Rearranged B-ALL. Nix MA, Mandal K, Geng H, Paranjape N, Lin YT, Rivera JM, Marcoulis M, White KL, Whitman JD, Bapat SP, Parker KR, Ramirez J, Deucher A, Phojanokong P, Steri V, Fattahi F, Hann BC, Satpathy AT, Manglik A, Stieglitz E, Wiita AP . Cancer discovery . Mar 16, 2021 An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike. Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbølle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, García-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A . Science (New York, N.Y.) . Nov 5, 2020 Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit. Zhang Y, Lu WJ, Bulkley DP, Liang J, Ralko A, Han S, Roberts KJ, Li A, Cho W, Cheng Y, Manglik A, Beachy PA . Proceedings of the National Academy of Sciences of the United States of America . Nov 2, 2020 Universal Polymerase Chain Reaction and Antibody Testing Demonstrate Little to No Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 in a Rural Community. Appa A, Takahashi S, Rodriguez-Barraquer I, Chamie G, Sawyer A, Duarte E, Hakim J, Turcios K, Vinden J, Janson O, Manglik A, Peluso MJ, Deeks SG, Henrich TJ, Torres L, Rodgers M, Hackett J, Chiu C, Havlir D, Greenhouse B . Open forum infectious diseases . Oct 30, 2020 Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Gordon DE, Hiatt J, Bouhaddou M, Rezelj VV, Ulferts S, Braberg H, Jureka AS, Obernier K, Guo JZ, Batra J, Kaake RM, Weckstein AR, Owens TW, Gupta M, Pourmal S, Titus EW, Cakir M, Soucheray M, McGregor M, Cakir Z, Jang G, O'Meara MJ, Tummino TA, Zhang Z, Foussard H, Rojc A, Zhou Y, Kuchenov D, Hüttenhain R, Xu J, Eckhardt M, Swaney DL, Fabius JM, Ummadi M, Tutuncuoglu B, Rathore U, Modak M, Haas P, Haas KM, Naing ZZC, Pulido EH, Shi Y, Barrio-Hernandez I, Memon D, Petsalaki E, Dunham A, Marrero MC, Burke D, Koh C, Vallet T, Silvas JA, Azumaya CM, Billesbølle C, Brilot AF, Campbell MG, Diallo A, Dickinson MS, Diwanji D, Herrera N, Hoppe N, Kratochvil HT, Liu Y, Merz GE, Moritz M, Nguyen HC, Nowotny C, Puchades C, Rizo AN, Schulze-Gahmen U, Smith AM, Sun M, Young ID, Zhao J, Asarnow D, Biel J, Bowen A, Braxton JR, Chen J, Chio CM, Chio US, Deshpande I, Doan L, Faust B, Flores S, Jin M, Kim K, Lam VL, Li F, Li J, Li YL, Li Y, Liu X, Lo M, Lopez KE, Melo AA, Moss FR, Nguyen P, Paulino J, Pawar KI, Peters JK, Pospiech TH, Safari M, Sangwan S, Schaefer K, Thomas PV, Thwin AC, Trenker R, Tse E, Tsui TKM, Wang F, Whitis N, Yu Z, Zhang K, Zhang Y, Zhou F, Saltzberg D, QCRG Structural Biology Consortium, Hodder AJ, Shun-Shion AS, Williams DM, White KM, Rosales R, Kehrer T, Miorin L, Moreno E, Patel AH, Rihn S, Khalid MM, Vallejo-Gracia A, Fozouni P, Simoneau CR, Roth TL, Wu D, Karim MA, Ghoussaini M, Dunham I, Berardi F, Weigang S, Chazal M, Park J, Logue J, McGrath M, Weston S, Haupt R, Hastie CJ, Elliott M, Brown F, Burness KA, Reid E, Dorward M, Johnson C, Wilkinson SG, Geyer A, Giesel DM, Baillie C, Raggett S, Leech H, Toth R, Goodman N, Keough KC, Lind AL, Zoonomia Consortium, Klesh RJ, Hemphill KR, Carlson-Stevermer J, Oki J, Holden K, Maures T, Pollard KS, Sali A, Agard DA, Cheng Y, Fraser JS, Frost A, Jura N, Kortemme T, Manglik A, Southworth DR, Stroud RM, Alessi DR, Davies P, Frieman MB, Ideker T, Abate C, Jouvenet N, Kochs G, Shoichet B, Ott M, Palmarini M, Shokat KM, García-Sastre A, Rassen JA, Grosse R, Rosenberg OS, Verba KA, Basler CF, Vignuzzi M, Peden AA, Beltrao P, Krogan NJ . Science (New York, N.Y.) . Oct 15, 2020 Evaluation of SARS-CoV-2 serology assays reveals a range of test performance. Whitman JD, Hiatt J, Mowery CT, Shy BR, Yu R, Yamamoto TN, Rathore U, Goldgof GM, Whitty C, Woo JM, Gallman AE, Miller TE, Levine AG, Nguyen DN, Bapat SP, Balcerek J, Bylsma SA, Lyons AM, Li S, Wong AW, Gillis-Buck EM, Steinhart ZB, Lee Y, Apathy R, Lipke MJ, Smith JA, Zheng T, Boothby IC, Isaza E, Chan J, Acenas DD, Lee J, Macrae TA, Kyaw TS, Wu D, Ng DL, Gu W, York VA, Eskandarian HA, Callaway PC, Warrier L, Moreno ME, Levan J, Torres L, Farrington LA, Loudermilk RP, Koshal K, Zorn KC, Garcia-Beltran WF, Yang D, Astudillo MG, Bernstein BE, Gelfand JA, Ryan ET, Charles RC, Iafrate AJ, Lennerz JK, Miller S, Chiu CY, Stramer SL, Wilson MR, Manglik A, Ye CJ, Krogan NJ, Anderson MS, Cyster JG, Ernst JD, Wu AHB, Lynch KL, Bern C, Hsu PD, Marson A . Nature biotechnology . Aug 27, 2020 Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms. Billesbølle CB, Azumaya CM, Kretsch RC, Powers AS, Gonen S, Schneider S, Arvedson T, Dror RO, Cheng Y, Manglik A . Nature . Aug 19, 2020 An ultra-potent synthetic nanobody neutralizes SARS-CoV-2 by locking Spike into an inactive conformation. Schoof M, Faust B, Saunders RA, Sangwan S, Rezelj V, Hoppe N, Boone M, Billesbølle CB, Puchades C, Azumaya CM, Kratochvil HT, Zimanyi M, Deshpande I, Liang J, Dickinson S, Nguyen HC, Chio CM, Merz GE, Thompson MC, Diwanji D, Schaefer K, Anand AA, Dobzinski N, Zha BS, Simoneau CR, Leon K, White KM, Chio US, Gupta M, Jin M, Li F, Liu Y, Zhang K, Bulkley D, Sun M, Smith AM, Rizo AN, Moss F, Brilot AF, Pourmal S, Trenker R, Pospiech T, Gupta S, Barsi-Rhyne B, Belyy V, Barile-Hill AW, Nock S, Liu Y, Krogan NJ, Ralston CY, Swaney DL, García-Sastre A, Ott M, Vignuzzi M, QCRG Structural Biology Consortium, Walter P, Manglik A . bioRxiv : the preprint server for biology . Aug 17, 2020 Universal PCR and antibody testing demonstrate little to no transmission of SARS-CoV-2 in a rural community. Appa A, Takahashi S, Rodriguez-Barraquer I, Chamie G, Sawyer A, Consortium C, Duarte E, Hakim J, Turcios K, Vinden J, Janson O, Manglik A, Peluso MJ, Deeks SG, Henrich TJ, Torres L, Rodgers M, Hackett J, Chiu C, Havlir D, Greenhouse B . medRxiv : the preprint server for health sciences . Aug 17, 2020 Author Correction: Structural insights into μ-opioid receptor activation. Huang W, Manglik A, Venkatakrishnan AJ, Laeremans T, Feinberg EN, Sanborn AL, Kato HE, Livingston KE, Thorsen TS, Kling RC, Granier S, Gmeiner P, Husbands SM, Traynor JR, Weis WI, Steyaert J, Dror RO, Kobilka BK . Nature . Aug 1, 2020 Bivalent binding of a fully human IgG to the SARS-CoV-2 spike proteins reveals mechanisms of potent neutralization. Wang B, Asarnow D, Lee WH, Huang CW, Faust B, Ng PML, Ngoh EZX, Bohn M, Bulkley D, Pizzorno A, Tan HC, Lee CY, Minhat RA, Terrier O, Soh MK, Teo FJ, Yeap YYC, Hu Y, Seah SGK, Maurer-Stroh S, Renia L, Hanson BJ, Rosa-Calatrava M, Manglik A, Cheng Y, Craik CS, Wang CI . bioRxiv : the preprint server for biology . Jul 15, 2020 β-Arrestin-Biased Angiotensin II Receptor Agonists for COVID-19. Manglik A, Wingler LM, Rockman HA, Lefkowitz RJ . Circulation . May 15, 2020 Agonist-selective recruitment of engineered protein probes and of GRK2 by opioid receptors in living cells. Stoeber M, Jullié D, Li J, Chakraborty S, Majumdar S, Lambert NA, Manglik A, von Zastrow M . eLife . Feb 25, 2020 Molecular Basis of Opioid Action: From Structures to New Leads. Manglik A . Biological psychiatry . Sep 12, 2019 Smoothened stimulation by membrane sterols drives Hedgehog pathway activity. Deshpande I, Liang J, Hedeen D, Roberts KJ, Zhang Y, Ha B, Latorraca NR, Faust B, Dror RO, Beachy PA, Myers BR, Manglik A . Nature . Jul 1, 2019 Development of an antibody fragment that stabilizes GPCR/G-protein complexes. Maeda S, Koehl A, Matile H, Hu H, Hilger D, Schertler GFX, Manglik A, Skiniotis G, Dawson RJP, Kobilka BK . Nature communications . Sep 13, 2018 Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor. Livingston KE, Mahoney JP, Manglik A, Sunahara RK, Traynor JR . eLife . Jun 22, 2018 Structure of the µ-opioid receptor-Gi protein complex. Koehl A, Hu H, Maeda S, Zhang Y, Qu Q, Paggi JM, Latorraca NR, Hilger D, Dawson R, Matile H, Schertler GFX, Granier S, Weis WI, Dror RO, Manglik A, Skiniotis G, Kobilka BK . Nature . Jun 13, 2018 A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action. Stoeber M, Jullié D, Lobingier BT, Laeremans T, Steyaert J, Schiller PW, Manglik A, von Zastrow M . Neuron . May 10, 2018 Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling. Staus DP, Wingler LM, Choi M, Pani B, Manglik A, Kruse AC, Lefkowitz RJ . Proceedings of the National Academy of Sciences of the United States of America . Mar 26, 2018 Yeast surface display platform for rapid discovery of conformationally selective nanobodies. McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC . Nature structural & molecular biology . Feb 12, 2018 Structural Basis for G Protein-Coupled Receptor Activation. Manglik A, Kruse AC . Biochemistry . Oct 10, 2017 Directed evolution of nanobody chaperones that stabilize GPCR:G-protein complexes. Aashish Manglik, Andrew C. Kruse, Antoine Koehl, Brian K. Kobilka, Aaron M. Ring . The FASEB Journal . Apr 1, 2017 Nanobodies to Study G Protein-Coupled Receptor Structure and Function. Manglik A, Kobilka BK, Steyaert J . Annual review of pharmacology and toxicology . Dec 7, 2016 Structure-based discovery of opioid analgesics with reduced side effects. Manglik A, Lin H, Aryal DK, McCorvy JD, Dengler D, Corder G, Levit A, Kling RC, Bernat V, Hübner H, Huang XP, Sassano MF, Giguère PM, Löber S, Scherrer G, Kobilka BK, Gmeiner P, Roth BL, Shoichet BK . Nature . Aug 17, 2016 Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation. Staus DP, Strachan RT, Manglik A, Pani B, Kahsai AW, Kim TH, Wingler LM, Ahn S, Chatterjee A, Masoudi A, Kruse AC, Pardon E, Steyaert J, Weis WI, Prosser RS, Kobilka BK, Costa T, Lefkowitz RJ . Nature . Jul 13, 2016 Allosteric coupling from G protein to the agonist-binding pocket in GPCRs. DeVree BT, Mahoney JP, Vélez-Ruiz GA, Rasmussen SG, Kuszak AJ, Edwald E, Fung JJ, Manglik A, Masureel M, Du Y, Matt RA, Pardon E, Steyaert J, Kobilka BK, Sunahara RK . Nature . Jun 29, 2016 Crystal structure of the human σ1 receptor. Schmidt HR, Zheng S, Gurpinar E, Koehl A, Manglik A, Kruse AC . Nature . Apr 4, 2016 Efficacy at the Mu Opioid Receptor: Insights from Orthosteric and Allosteric Ligands. Kathryn E Livingston, Jacob Mahoney, Aashish Manglik, Brian Kobilka, Roger Sunahara, John R Traynor . The FASEB Journal . Apr 1, 2016 Structural Basis for Allosteric Coupling Between G Protein and the Agonist-Binding Pocket in GPCRs. Jacob Mahoney, Brian DeVree, Gisselle Velez-Ruiz, Soren Rasmussen, Adam Kuszak, Elin Edwald, Aashish Manglik, Matthieu Masureel, Yang Du, Rachel Matt, Els Pardon, Jan Steyaert, Brian Kobilka, Roger Sunahara . The FASEB Journal . Apr 1, 2016 Abstract B101: Engineered PD-1 variants as immunotherapies for cancer. Sydney R. Gordon, Roy Maute, Aaron Mayer, Melissa McCracken, Arutselvan Natarajan, Nan Guo, Richard Kimura, Jonathan M. Tsai, Aashish Manglik, Andrew Kruse, Sanjiv Gambhir, Irving L. Weissman, Aaron M. Ring . Imaging and Targeting of the Antitumor Response . Jan 1, 2016 High-density grids for efficient data collection from multiple crystals. Baxter EL, Aguila L, Alonso-Mori R, Barnes CO, Bonagura CA, Brehmer W, Brunger AT, Calero G, Caradoc-Davies TT, Chatterjee R, Degrado WF, Fraser JS, Ibrahim M, Kern J, Kobilka BK, Kruse AC, Larsson KM, Lemke HT, Lyubimov AY, Manglik A, McPhillips SE, Norgren E, Pang SS, Soltis SM, Song J, Thomaston J, Tsai Y, Weis WI, Woldeyes RA, Yachandra V, Yano J, Zouni A, Cohen AE . Acta crystallographica. Section D, Structural biology . Jan 1, 2016 Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging. Maute RL, Gordon SR, Mayer AT, McCracken MN, Natarajan A, Ring NG, Kimura R, Tsai JM, Manglik A, Kruse AC, Gambhir SS, Weissman IL, Ring AM . Proceedings of the National Academy of Sciences of the United States of America . Nov 10, 2015 Structural Insights into the Dynamic Process of ß2-Adrenergic Receptor Signaling. Aashish Manglik, Tae Hun Kim, Matthieu Masureel, Christian Altenbach, Zhongyu Yang, Daniel Hilger, Michael T. Lerch, Tong Sun Kobilka, Foon Sun Thian, Wayne L. Hubbell, R. Scott Prosser, Brian K. Kobilka . Cell . Sep 1, 2015 Propagation of conformational changes during μ-opioid receptor activation. Sounier R, Mas C, Steyaert J, Laeremans T, Manglik A, Huang W, Kobilka BK, Déméné H, Granier S . Nature . Aug 5, 2015 Structural insights into µ-opioid receptor activation. Huang W, Manglik A, Venkatakrishnan AJ, Laeremans T, Feinberg EN, Sanborn AL, Kato HE, Livingston KE, Thorsen TS, Kling RC, Granier S, Gmeiner P, Husbands SM, Traynor JR, Weis WI, Steyaert J, Dror RO, Kobilka BK . Nature . Aug 5, 2015 SIGNAL TRANSDUCTION. Structural basis for nucleotide exchange in heterotrimeric G proteins. Dror RO, Mildorf TJ, Hilger D, Manglik A, Borhani DW, Arlow DH, Philippsen A, Villanueva N, Yang Z, Lerch MT, Hubbell WL, Kobilka BK, Sunahara RK, Shaw DE . Science (New York, N.Y.) . Jun 19, 2015 Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling. Manglik A, Kim TH, Masureel M, Altenbach C, Yang Z, Hilger D, Lerch MT, Kobilka TS, Thian FS, Hubbell WL, Prosser RS, Kobilka BK . Cell . May 14, 2015 A comparison of chemical shift sensitivity of trifluoromethyl tags: optimizing resolution in ¹⁹F NMR studies of proteins. Ye L, Larda ST, Frank Li YF, Manglik A, Prosser RS . Journal of biomolecular NMR . Mar 27, 2015 G Protein-coupled Receptor Kinases of the GRK4 Protein Subfamily Phosphorylate Inactive G Protein-coupled Receptors (GPCRs). Li L, Homan KT, Vishnivetskiy SA, Manglik A, Tesmer JJ, Gurevich VV, Gurevich EV . The Journal of biological chemistry . Mar 13, 2015 Goniometer-based femtosecond crystallography with X-ray free electron lasers. Cohen AE, Soltis SM, González A, Aguila L, Alonso-Mori R, Barnes CO, Baxter EL, Brehmer W, Brewster AS, Brunger AT, Calero G, Chang JF, Chollet M, Ehrensberger P, Eriksson TL, Feng Y, Hattne J, Hedman B, Hollenbeck M, Holton JM, Keable S, Kobilka BK, Kovaleva EG, Kruse AC, Lemke HT, Lin G, Lyubimov AY, Manglik A, Mathews II, McPhillips SE, Nelson S, Peters JW, Sauter NK, Smith CA, Song J, Stevenson HP, Tsai Y, Uervirojnangkoorn M, Vinetsky V, Wakatsuki S, Weis WI, Zadvornyy OA, Zeldin OB, Zhu D, Hodgson KO . Proceedings of the National Academy of Sciences of the United States of America . Oct 31, 2014 Covalent agonists for studying G protein-coupled receptor activation. Weichert D, Kruse AC, Manglik A, Hiller C, Zhang C, Hübner H, Kobilka BK, Gmeiner P . Proceedings of the National Academy of Sciences of the United States of America . Jul 8, 2014 The role of protein dynamics in GPCR function: insights from the β2AR and rhodopsin. Manglik A, Kobilka B . Current opinion in cell biology . Feb 17, 2014 Activation and allosteric modulation of a muscarinic acetylcholine receptor. Kruse AC, Ring AM, Manglik A, Hu J, Hu K, Eitel K, Hübner H, Pardon E, Valant C, Sexton PM, Christopoulos A, Felder CC, Gmeiner P, Steyaert J, Weis WI, Garcia KC, Wess J, Kobilka BK . Nature . Nov 20, 2013 Applications of molecular replacement to G protein-coupled receptors. Kruse AC, Manglik A, Kobilka BK, Weis WI . Acta crystallographica. Section D, Biological crystallography . Oct 18, 2013 Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody. Ring AM, Manglik A, Kruse AC, Enos MD, Weis WI, Garcia KC, Kobilka BK . Nature . Sep 22, 2013 The role of ligands on the equilibria between functional states of a G protein-coupled receptor. Kim TH, Chung KY, Manglik A, Hansen AL, Dror RO, Mildorf TJ, Shaw DE, Kobilka BK, Prosser RS . Journal of the American Chemical Society . Jun 14, 2013 Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide. Shukla AK, Manglik A, Kruse AC, Xiao K, Reis RI, Tseng WC, Staus DP, Hilger D, Uysal S, Huang LY, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis WI, Kossiakoff AA, Kobilka BK, Lefkowitz RJ . Nature . Apr 21, 2013 Corrigendum: A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins. Pil Seok Chae, Søren G. F. Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, Samuel H. Gellman . Chemistry - A European Journal . Apr 15, 2013 Crystal structure of active Beta-arrestin1 bound to phosphorylated carboxy-terminus of a G protein-coupled receptor. Arun K. Shukla, Aashish Manglik, Andrew Kruse, Rosana Reis, Wei Chou Tseng, Dean P. Staus, Daniel Hilger, Serdar Uysal, LiYin Huang, Marcin Peduch, Prachi Tripathi-Shukla, Akiko Koide, Shohei Koide, William I. Weis, Anthony K. Kossiakoff, Brian K. Kobilka, Robert J. Lefkowitz . The FASEB Journal . Apr 1, 2013 The dynamic process of β(2)-adrenergic receptor activation. Nygaard R, Zou Y, Dror RO, Mildorf TJ, Arlow DH, Manglik A, Pan AC, Liu CW, Fung JJ, Bokoch MP, Thian FS, Kobilka TS, Shaw DE, Mueller L, Prosser RS, Kobilka BK . Cell . Jan 31, 2013 Role of detergents in conformational exchange of a G protein-coupled receptor. Chung KY, Kim TH, Manglik A, Alvares R, Kobilka BK, Prosser RS . The Journal of biological chemistry . Aug 14, 2012 Inside Cover: A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins (Chem. Eur. J. 31/2012). Pil Seok Chae, Søren G. F. Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, Samuel H. Gellman . Chemistry - A European Journal . Jul 27, 2012 A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins. Chae PS, Rasmussen SG, Rana RR, Gotfryd K, Kruse AC, Manglik A, Cho KH, Nurva S, Gether U, Guan L, Loland CJ, Byrne B, Kobilka BK, Gellman SH . Chemistry (Weinheim an der Bergstrasse, Germany) . Jun 22, 2012 Structure of the δ-opioid receptor bound to naltrindole. Granier S, Manglik A, Kruse AC, Kobilka TS, Thian FS, Weis WI, Kobilka BK . Nature . May 16, 2012 Crystal structure of the µ-opioid receptor bound to a morphinan antagonist. Manglik A, Kruse AC, Kobilka TS, Thian FS, Mathiesen JM, Sunahara RK, Pardo L, Weis WI, Kobilka BK, Granier S . Nature . Mar 21, 2012 Stably integrated luxCDABE for assessment of Salmonella invasion kinetics. Flentie KN, Qi M, Gammon ST, Razia Y, Lui F, Marpegan L, Manglik A, Piwnica-Worms D, McKinney JS . Molecular imaging . Sep 1, 2008
